![]() ![]() Specifically associated with regulation of WalR function was Was hypothesized, and whether the potential AS walR was In the present study, a potential AS walR Identical) with the homologous vicR gene in S. faecalis walR gene withīLAST searches, a high similarity (the DNA sequences are 70% Similar structural and functional relationships between vicRĪdditionally, by mapping the E. Related Gram-positive cocci, it was suggested that there were With different levels of vicR antisense RNA and that theīiofilm biomass decreased in the vicR antisense Was found that the production of VicR protein associated inversely VicR antisense RNA transcript has been identified in S. Transcriptomics analyses, AS RNA regulators can be identified in Transcription or translation and performs regulatory functions This AS RNA-induced duplex complex generally inhibits mRNA Target messenger RNA (mRNA) by base-pairing, and their interaction Was essential for bacterial viability ( 4).Ī noncoding antisense RNA (AS RNA) can bind to the WalR deletion mutants indicated that this regulatory gene Scaffold contributes to the failure of persistent infected rootįaecalis, it was reported that the inability to construct faecalis to form the three-dimensional biofilm Bacterial growth and biofilmĪggregation are strongly associated with the process of humanĪbility of E. The essential gene walR, isĬlosely associated with bacterial growth, virulence and biofilmĮxtracellular polysaccharide synthesis and aggregation ( 1). Proposed because of its major roles in the regulation of genesĪssociated with cell wall synthesis and biofilm formation ( 7). Genera, such as Staphylococcus aureus, Enterococcusįaecalis, and Streptococcus mutans ( S. Gram-positive bacteria and is annotated as VicRK or YycFG in some Originally from Bacillus subtilis is highly conserved in The WalRK (also known as VicRK or YycFG) TCS Stress, antibiotic pressure and nutrient starvation, and relays theĪctivated phosphoryl group to the response regulator for geneĮxpression modulation by binding at the regulatory regions of Recognition of environmental stimuli, including pH, oxidative ![]() Receptor and its cognate response regulator ( 6). Typical TCS consists of a histidine sensory kinase membrane Viability, virulence, biofilm formation, quorum sensing and Systems are involved in various cellular processes, including cell Regulatory regions of target genes accordingly ( 4). The expression of virulence genes in response to microenvironmentalĬonditions, including the host environment, and binds to the Sense and adapt to varying environmental stresses duringĭuring long-term interaction with the host, the two-component (or commensal) bacterial pathogens can switch from the commensal faecalis), a generally commensal organism, hasĮmerged as the major pathogen for persistent periapical Collectively, the data suggest a role for AS walR as a post‑transcriptional modulator of the WalR regulator in E. faecalis. In summary, the present study detected a novel antisense walR RNA that leads to a reduction in biofilm formation and the pathogenicity of E. faecalis. Furthermore, the pathogenicity of E. faecalis was markedly decreased by AS walR overexpression in an in vivo periapical periodontitis model. It was showed that overexpression of AS walR leads to reduced biofilm formation and exopolysaccharide synthesis. The levels of antisense walR RNA transcripts were inversely associated with the production of WalR protein. The present study detected and confirmed a 550‑bp noncoding antisense RNA with the potential to attenuate the activities of the essential response regulator WalR. AS walR overexpression mutants were constructed, and the biofilm biomass was determined using a crystal violet microtiter assay. Northern blotting and 5'‑rapid amplification of cDNA ends (5'‑RACE) assays were conducted to detect and confirm a novel walR antisense (AS walR) RNA. Adjacent downstream genes walK, EF1195, EF1196, and EF1197 were co‑transcribed and detect antisense walR RNA. Reverse transcription‑PCR assays were performed to validate walR. The aim of the present study was to investigate the role of antisense walR RNA in the regulation of adjacent downstream genes. Enterococcus faecalis ( E. faecalis) is regarded as the major pathogen for persistent periapical periodontitis. ![]()
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